Bacterial strains

Bacterial strains. BL21(DE3). F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3). - an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene ...
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Microbiology

Bacterial strains

BL21(DE3) F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3) - an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq - Transformed plasmids containing T7 promoter driven expression are repressed until IPTG induction of T7 RNA polymerase from a lac promoter. BL21 (DE3) pLysS F- ompT gal dcm lon hsdSB(rB- mB-) λ(DE3) pLysS(cmR) - pLys plasmid chloramphenicol resistant; grow with chloramphenicol to retain plasmid - Chloramphenicol resistant - The pLysS plasmid encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression from transformed T7 promoter containing plasmids when not induced. DB3.1 F- gyrA462 endA1 glnV44 ∆(sr1-recA) mcrB mrr hsdS20(rB-, mB-) ara14 galK2 lacY1 proA2 rpsL20 xyl5 ∆leu mtl1 - useful for propagating plasmids containing the ccdB gene (gyrA462 ). - streptomycin resistant DH5α F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80lacZ∆M15 ∆(lacZYA-argF)U169, hsdR17(rK- mK+), λ– - nalidixic acid resistant DH10B (Invitrogen) F- endA1 recA1 galU galK deoR nupG rpsL ∆lacX74 Φ80lacZ∆M15 araD139 ∆(ara,leu)7697 mcrA ∆(mrr-hsdRMS-mcrBC) λ- suitable for cloning methylated cytosine or adenine containing DNA - Streptomycin resistant

Microbiology 2

Microbiology JM109 endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ ∆(lac-proAB) glnV44 e14- [F' traD36 proAB+ lacIq lacZ∆M15] hsdR17(rK- mK+) - Partly restriction-deficient; good strain for cloning repetitive DNA (RecA–). - nalidixic acid resistant SURE (Stratagene) endA1 glnV44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5 uvrC e14- ∆(mcrCBhsdSMR-mrr)171 F'[ proAB+ lacIq lacZ∆M15 Tn10] - increased stability for inverted repeats and Z-DNA - nalidixic acid resistant - kanamycin resistant - tetracycline resistant SURE2 (Stratagene) endA1 glnV44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5 uvrC e14- ∆(mcrCBhsdSMR-mrr)171 F'[ proAB+ lacIq lacZ∆M15 Tn10 Amy CmR] - increased stability for inverted repeats and Z-DNA - nalidixic acid resistant - kanamycin resistant - tetracycline resistant - chloramphenicol resistant for < 40 µg/ml, sensitive for > 100 µg/ml XL1-Blue (Stratagene) endA1 gyrA96 thi-1 recA1 relA1 lac glnV44 F'[ ::Tn10 proAB+ lacIq ∆lacZM15] hsdR17(rKmK+) - nalidixic acid resistant - tetracycline resistant SW102 F- endA1 recA1 ∆galK deoR nupG rpsL ∆lacX74 Φ80lacZ∆M15 araD139 ∆(ara,leu)7697 mcrA ∆(mrr-hsdRMS-mcrBC) [ λcI857 (cro-bioA) ] - Streptomycin resistant

Microbiology 3