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BIONISIS OPLC
The key to higher
throughput
Ref 9001-3/00 - Printed in France, Specifications subject to change without notice.
in the world of liquid chromatography
BIONISIS
OPLC
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BIONISIS OPLC Optimum Performance Laminar Chromatography Bionisis is committed to bringing a new concept in liquid chromatography to industrial and academic laboratories. We recognize the need for high throughput, high resolution and low cost. We are dedicated to providing you with solutions and supporting your efforts in implementing OPLC, a major advance
Bionisis OPLC 50 system
in liquid chromatography.
A Bionisis OPLC system in your laboratory means increased productivity through…
Simultaneous multi-sample processing Fast separation ( av. 10 to 20 min ) Reduced sample preparation Low solvent consumption ( < 5ml/run )
The real key to higher throughput OPLC … Optimum Performance Laminar Chromatography
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OPLC 50 A sturdy, robust system reliable and easy to service Cassette with HTSorb™ chromatographic sorbent
User-friendly programming • Programs run with a limited number of parameters • System set-up in a minute
• Choice of chromatographic techniques (reverse phase, normal phase …) • Selection of particle sizes according to resolution required • No clogging (even with dirty samples), no phantom peaks
Easy solvent change • Flexible solvent composition programming
Low solvent consumption • Only 5 ml of solvent for a complete run involving up to 70 samples at a time
Process up to 70 samples simultaneously OPLC … Optimum Performance Laminar Chromatography
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BIONISIS OPLC An efficient, robust technique OPLC is a fast and reliable liquid chromatography technique, ideal for laboratories faced with the problem
Separations on 5 µm particles for reproducible separations
of processing a large number of complex mixtures of chemical and biochemical compounds. In OPLC,
the sorbent flat bed is completely covered by a
flexible Teflon® membrane and maintained under pressure in a vapor-free state. The eluent is pushed across the flat sorbent bed at a controlled flowrate. Bionisis OPLC separations are carried out on HTSorb™ «flat» columns. Since the chromatographic support is supplied in ultra-thin (200µm) layers, it is possible to use 3 µm particles with
Two dimensional separations for complex compounds
very limited build-up of back pressure. OPLC provides a wide choice of established detection methods (UV, visible, fluorescence…) plus selective visualisation of compounds. The linear migration of the solvent front makes OPLC an extremely efficient technique. With an HTSorb 5 µm sorbent bed, typical separation time is from 5 to 30 minutes. Analytical or preparative mode with on-line fraction collection
The ultimate in simplicity
1 2 3
Automatically deposit sample on the HTSorb column Load the column into the separation unit Start the program
Separations in 15 minutes ! OPLC … Optimum Performance Laminar Chromatography
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PHARMACEUTICAL FOOD
The perfect complement to HPLC in your laboratory
AND BEVERAGE
COSMETICS ENVIRONMENTAL
HTSorb™ silica 11 µm Development time : 18 min
CLINICAL
280 nm
RESEARCH
urine 5 urine 4 0.01 µg risperidone 0.1 µg risperidone 1 µg risperidone
AU
0.01 µg clozapine
200 180 160 140 120 100 80 60 40 20 0
0.1 µg clozapine 1 µg clozapine
Sorbent : HTSorb™ HP 5 µm silica Development time (with overrun) : 17 min Sample : 50 µg main component spiked with 0.5 µg of each known impurity (no. 1-4),band size : 10 mm Detection : absorbance at 233 nm
233 nm
urine 3 blank urine 2
AU
urine 1
20
30
40
50
60
70
80
90
100 110 120 130 140 150mm
Psychotropic drugs in urine
100
Personal communication, E.N. Papp and E. Nagy
Sorbent : HTSorb™ HP 5 µm silica Development time (with prewash) : 19.3 min Sample : wheat extract (spiked with 5-5 ng/g B1 B2, G1, G2) Fluorescent detection
0 0
50
100
150
mm
Impurity test (main component : phthaloyl-amlodipine) Prepared in Gedeon Richter Co. Ltd. Z. Végh and K. Ferenczi-Fodor
4
50
xylose
galactose glucose fructose
raffinose
1 2
0
maltose saccharose
Sorbent : HTSorb™ 11 µm silica
3
mm Standards
Aflatoxins in wheat Personal communication,E. Papp and K.H. –Otta Plant extract A
Plant extract B
Plant extract C 0
50
100
150
mm
Sugar separation Personal communication, E. Sárdi
OPLC … Optimum Performance Laminar Chromatography
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BIONISIS OPLC
Specifications Separation chamber
Dimensions : 39 (L) x 29.6 (W) x 13.5 cm (H) - Weight : 19 kg External pressure : 5 MPa max
Delivery system
Dimensions : 40 (L) x 20.6 (W) x 20 (H) cm - Weight : 11 kg Power input rate : 130 VA Power requirements : 110/220 V, 50HZ Hydraulic liquid supply ; max external pressure : 92 ml/min., 5 MPa Eluent flow range, max eluent pressure : 10-10 000 µl/min., 4.5 MPa Reproducibility of eluent delivery : 0.1%
Sorbent layers for HTSorb™ flat columns Ready-to-use, pre-sealed 200 x 200 x 0.2 mm or 200 x 100 x 0.2 mm aluminum foils HTSorb™ HP HTSorb™ RP 18 HTSorb™
silica, 5 µm particle size silica, 11 µm particle size reverse phase silica, 11 µm particle size
Distributed by
BIONISIS
OPLC
Parc Technologique du Plessis Robinson, Bâtiment "Le Carnot", 18, avenue Edouard Herriot, 92350 Le Plessis Robinson, France Tel 33 (0)1 40 83 82 00 • Fax 33 (0)1 40 83 82 01 • www.bionisis.com •
[email protected]
Ref 9001-3/00 - Printed in France, Specifications subject to change without notice.
Optimum Performance Laminar Chromatography OPLC and HTSorb are trademarks of Bionisis, S.A., France. Teflon is a registered trademark of Dupont de Nemours
