Course timing Morning

Day 1

th

28 Nov

Subjects -

Opening

-

Lectures: + Replication of rabies virus; + Rabies phosphoprotein P; + Rabies P3 protein ;

Afternoon Professors

Subjects

Professors

Danielle

Mutation-Amplification PCR1

Danielle

Blondel

and PCR2 of P3 and p3m by PCR

Blondel

PCR3 and Purification of products

Danielle

by agarose gel

Blondel

+Presentation of the practical course ; PCR method to introduce mutation 2

nd

29 Nov 3

Poliovirus : Molecular Biology and Pathogenesis of Poliomyelitis

Bruno Blondel

rd

30 Nov

- Digestion of PCR products by Real time quantitative PCR: principe and

Jean Luc

applications

Guesdon

restriction enzymes - Digestion of the cloning vector - Purification of the digested

Danielle Blondel

vector by agarose gel 4th 1Dec

Molecular Epidemiology

Le Thi Kim Tuyen

- Ligation of the inserts in the digested vector -Transformation of bacteria

5 th

Culture of colonies on LB

2 Dec

ampicilline plates during the nigh Diagnosis of RNA viruses cause human pathogen

Quynh Mai

at 37oC Week-End: put the bacteria

Danielle Blondel

Danielle Blondel

at 4°C th

6

5 Dec

- Identification of the positive Minipreparation and digestion of miniprep with

colonies by agarose gel

Danielle

restriction enzymes

- Amplification of the positive

Blondel

colonies in LB solution 7th

Diagnosis of RNA viruses that cause pathogen in

6 Dec

domestic animals may be transmitted to humans

8

th

7 Dec 9

th

8 Dec

Laboratory technique for elimination strategy and

Tien Dung Hanh Phuc

Purification of plasmids

Blondel Transfection

surveillance of measles in Vietnam Diagnosis of HCV, HBV and blood control for

Danielle Danielle Blondel

Khanh Hoa

transfusion

Replication of RNA viruses Reverse genetics system of RNA viruses

th

10

-

Rabies situation in Vietnam

Nguyen Hong

- Fixation of the cells and analysis

9 Dec

-

Cell culture of rabies vaccine for human use

Hanh

by fluorescene - Closing

Danielle Blondel