Reverse Phase HPLC is employed for the initial analysis and the final large scale purification of peptides. The first step of the production of ... chromatogram.
Standard Peptide Mixture Precise and Fast Equilibration Time Method Conditions Column: Catalog No.: Dimensions: Solvents:
Cogent Bidentate C8 300™ 5µm, 300Å. 40008-75P-3M 4.6 x 75 mm A: DI water + 0.1% trifluoroacetic acid (TFA) B: acetonitrile + 0.1% TFA Gradient: Time (min) %B 0.0 9 5.0 21 20.0 27 21.0 9 Post Tme: 5 min Flow Rate: 1.0 mL/min Sample Prep: 1. Gly-Tyr 2. Val-Tyr-Val 3. Methionine enkephalin 4. Angiotensin II 5. Leucine enkephalin Detection: UV 214 nm.
Discussion HPLC in various modes is a main technique for the characterization of peptides. Reverse Phase HPLC is employed for the initial analysis and the final large scale purification of peptides. The first step of the production of synthetic peptides usually involves an initial separation of the peptides in the mixture on an analytical scale. Next the purification and collection of the target peptide follows. The Figure shown here presents the use of RP-HPLC with a Cogent Bidentate C8 300™ column in the separation of a five peptide mixture. The 300Å pore size of the sorbent is ideal for separation of small peptides chosen for the presented chromatogram. This column is a great choice for the gradient analysis of these due to the very fast equilibration time between injections (less than 5 minutes). For more information visit www.MTC-USA.com
Mass Spec: Atmospheric Pressure. Chemical Ionization in the. Positive mode: APCI+. Discussion: Major Peaks. Table below: Major peaks in mass spectra of ...
Chromatogram C shows the retention and elution of. Carvone in organic ... Bidentate C18⢠column, a quick âstep gradientâ or change of mobile ... âPrep upâ in NP and âCheck Purityâ in RP on the Same Analytical Column. HPLC. 03-H-08-A1.
ESI â neg - Agilent 6210 MSD TOF mass spectrometer. Discussion ... depending on individual instrument configuration. It is important to remember not to ...
The upper chromatogram is the initial separation, ... conditions that would hydrolyze other reversed phases. See next page for ... silica in reversed phase.
Description. APP A44. APPLICA. TION. NOTES. Method Conditions. Column: ... retention higher percentages of water in the mobile phase can be used. The diminished hydrophobicity is shown in Table 1. For more information visit www.
as base) bound to a ribose or deoxyribose sugar. Examples of these include cytidine, uridine, adenosine, guanosine, thymidine and inosine. Nucleosides can ...
Discussion. The polar compound, Metformin, and the nonpolar compound. Glyburide, can be retained on the same stationary phase (see A. & B), utilizing the ...
40018-75P. Cogent Bidentate⢠C18 HPLC Column, 4µm \,100A 4.6mm x 75mm. Cat. ... Solvent A: 100% DI water + ... 1mg of the compound dissolved in 1 mL.
for melamine: - Agilent 6210 MSD TOF mass spectrometer. Note: When an instrument with ... Use of LC-MS detection eliminates the need for derivatization (as is ...
Mass Spec: Atmospheric Pressure. Chemical Ionization in positive mode: APCI+ Single Ion Monitoring. Discussion. Two proprietary compounds, which are ...
Cholesterol⢠HPLC columns, peaks ETES and ESTN are resolved with polarity-based, Reverse Phase, ACN as the organic modifier. ⢠With other C18 columns, ...
This active pharmaceutical ingredient was separated from related compounds in a real life pharmaceutical preparation. Note the excellent peak shape and the ...
Cogent Diamond Hydride⢠HPLC Column, 100à , 4µm, 2.1 x 150mm. Cat. No. ... The analysis of succinic acid is mostly done using gas chromatography. (GC) ...
functions, reduction of cholesterol level and reduction of the risk of cancer and the mprovement of digestion/absorption of food ingredients such as minerals.
Cogent Silica-C⢠HPLC Column, 100à , 4µm, 4.6 x 75 mm. Cat. No. Description ... determination of amino acids in many types of samples. Ordinary columns ...
Cogent Bidentate C18 HPLC Column, 100à , 4µm, 4.6 x 75 mm, Standard End Fittings. Cat. No. ... refraction indices between solvent A and solvent B in gradient ...
