pH changing when moving to the acidic range. Start with the CElixir⢠Accelerator Solution (B) pH 2.5. CElixirâ¢. Accelerator Solution is used as your run buffer ...
CElixir™ uniquely offers stable EOF from pH 1-11. CElixir™ is easy to use. CElixir™ produces EOF differently on bare fused silica capillaries. New Methodology must be found for your current CZE Methods.
CElixir™ is optimized for analytes that are positively charged or amphoteric substances. Until now, CZE has been very erratic in this range due to the limitations of using bare fused silica as the source of EOF. For this reason, many scientists have developed CZE methods at a pH that is higher than optimal for their sample matrix. CElixir™ offers major advantages, when utilized in our recommended optimization procedures. These result in benefits to you, since you can run your method at low pH (1-7) without the variability of EOF. Your methods can now be component dictated rather than capillary dictated. It is recommended that when developing your method with CElixir™ you scout for your optimal pH. The first step will be to get a peak and determine the EOF using CElixir™. The EOF you determine, even in the basic pH range, will be very close to the EOF in the acidic range. This is an advantage to the practitioner in that it is easy to get a peak in the basic range and not have to worry about pH changing when moving to the acidic range. Start with the CElixir™ Accelerator Solution (B) pH 2.5. CElixir™ Accelerator Solution is used as your run buffer and to solubilize and prepare your samples before injection. If your samples do not go into solution or if they precipitate out at this pH, you should either select another pH or add organic solvent to the sample matrix. See the CElixir™ Operating and Trouble Shooting Manual for guidance on this. Be sure to add a neutral marker to the injection for EOF determination. Follow the instructions in the CElixir™ Operating and Trouble Shooting Manual or the HOW TO USE CElixir™ Easy To Apply Instructions Card to condition your capillary and run your CZE with CElixir™. Once you have a peak (usually a peak is found on the first run) determine if you have good resolution and the EOF from the migration time of the neutral marker. Then try pH CElixir™ with Accelerator Solution (B) pH 4.2, 6.2, 8.2, then 9.2. Determine which two pH’s you have the best resolution between. It is likely that your best performance will be between these two pHs. You can optimize your peak area, shape and resolution by adjusting the pH between these two by following the instructions on page 24 of the CElixir™ Operating and Trouble Shooting Manual on CElixir™ pH Adjustment.
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It is imperative that you do not take your current method and simply replace the run buffer with CElixir™. The separation mechanisms and solubility parameters are very different than in normal CZE and your results will be dramatically different.
Vials# 29, 31 â CE grade Water. 4. Vial# 32 â CElixir ... Stop Collecting data at the end of your run, for example 15.0 minutes. Rinse. 10. ... 11. Using pressure, run CEwater through the capillary using Inlet Vial#29 and Outlet. Vial# 10 for 0.5
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tion There is no need to filter any of the CElixir Solution (A) before coating ... celerator Solution (B) can be used as your sample diluent but you must be careful to ...
Welcome to the PQ Kit! These instructions are designed to help you to quickly familiarize yourself with the procedures needed to fully qualify your HPLC using ...
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