Vials# 29, 31 â CE grade Water. 4. Vial# 32 â CElixir ... Stop Collecting data at the end of your run, for example 15.0 minutes. Rinse. 10. ... 11. Using pressure, run CEwater through the capillary using Inlet Vial#29 and Outlet. Vial# 10 for 0.50 ...
Sample Method for Using CElixir with a Beckman P/ ACE 5000 Series Capillary Electrophoresis Instrument Easy to Apply Instructions It is very easy to set up a method in the Beckman P/ACE 5000 series instrument. Using the following parameters, fill your vials and enter this information into instrument for best results. Fill your vials as follows 1. 2. 3. 4. 5. 6.
Vial# 10 – waste Vials# 4, 33, 34 – CElixir Accelerator (B) with the pH of your choice Vials# 29, 31 – CE grade Water Vial# 32 – CElixir Initiator (A) Vial# 30 – 0.1 NaOH Other Vials – Samples
Starting with a new capillary 1. Rinse the capillary using pressure for 5 minutes with 0.1N NaOH. 2. Rinse the capillary using pressure for 1 minute with CEwater vial# 29.
Coating the Capillary 3. With pressure, rinse the capillary from vial#32 with the outlet vial# 10 (forward
high 20 p.s.i.) for 0.20 minute. This is the Initiator (A) Step.
4. With pressure, rinse the capillary from vial#33 with the outlet vial#10 (forward
high 20 p.s.i.) for 0.50 minute. This is the first Accelerator (B) Step for coating.
Inject Your Sample 5. Inject your sample using pressure for 5 seconds with the outlet vial#4. 6. Inject a “Water Plug” behind your sample. For 1.0 second, using pressure, inject
CEwater behind your sample for injection precision and a possible stacking effect. Inlet is Vial#31 and outlet vial is #4.
Separate 7. Run your separation, for example 25kV for 15.0 minutes to be adapted in function
of capillary length and observed µAmp. It is important to separate using inlet Vial#34 and the outlet Vial#4 during the run. Use 1 minute ramping time. 8. Use the AutoZero function for example after 2 minutes. 9. Stop Collecting data at the end of your run, for example 15.0 minutes. Rinse 10. Using pressure, run 0.1N NaOH through the capillary using Inlet Vial#30 and
Outlet Vial# 10 for 0.50 minutes (Forward High)
11. Using pressure, run CEwater through the capillary using Inlet Vial#29 and Outlet
Vial# 10 for 0.50 minutes (Forward High)
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