(min) Time (min) P/E - eLife Sciences

1000. 2000. 3000. 4000. 5000. Time (min). Time (min). Time (min). Time (min). Time (min). Time (min). Time (min). Time (min). Time (min). Time (min). P/E (p ro.
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30 20 10 0

0

10

20

30

Phosphorylated IGF1R-kin P/E (product/enzyme)

P/E (product/enzyme)

Unphosphorylated IGF1R-kin 40

2000 1500 1000 500 0 0.0

Unphosphorylated IGF1R-jmk

25 20 15 10 5 0 0.0

2.5

5.0

7.5

10.0

7.5 5.0 2.5 0.0 20

30

P/E (product/enzyme)

P/E (product/enzyme)

10.0

1750 1500 1250 1000 750 500 250 0 0.0

750 500 250 0 20

30

P/E (product/enzyme)

P/E (product/enzyme)

1000

10

200

100

0 0.0

500

10

20

Time (min)

30

P/E (product/enzyme)

P/E (product/enzyme)

Unphosphorylated IGF1R-fl + IGF1

0

2.5

5.0

7.5

Phosphorylated IGF1R-fl

5000 4000 3000 2000 1000 0 0

10

20

30

Time (min)

1000

0

7.5

300

Time (min)

1500

5.0

Time (min)

Unphosphorylated IGF1R-fl

0

2.5

Phosphorylated IGF1R-icd

Time (min)

1250

10.0

Time (min)

Unphosphorylated IGF1R-icd

10

7.5

Phosphorylated IGF1R-jmk

Time (min)

0

5.0

Time (min)

P/E (product/enzyme)

P/E (product/enzyme)

Time (min)

2.5

Phosphorylated IGF1R-fl + IGF1

5000 4000 3000 2000 1000 0

0

10

20

Time (min)

30