Use the Synergy of Pressure, Chemistry and Mechanical Force to Optimize Yield. ⢠Standardize Extraction of Proteins from C. elegans by Pressure Cycling.
ProteoSolve-CE Native A Pressure-Enhanced Kit for the Extraction of Native Protein from C. elegans The nematode Caenorhabditis elegans is so resistant to lysis that specimens aboard the Space Shuttle Columbia were the only survivors of the disastrous crash [1]. However, using the ProteoSolve-CE Native Kit in combination with The PCT Shredder system and pressure cycling technology (PCT) results in nearly total disruption of the nematodes, including resilient larval stages and worm embryos. The ProteoSolve-CE Native Kit was developed for the maximal recovery of Native proteins and protein complexes from the nematode C. elegans under minimally denaturing conditions in which Native conformation and activity are preserved. Protein yields are greater than those obtained from bead beating or processing with mortar and pestle in the presence of liquid nitrogen. Furthermore, Smejkal et al. [2] showed that bead beating may result in protein loss through denaturation and aggregation.
PCT Shredder (+) PCT
LNP
Extraction of Protein from C. elegans Using PCT in Combination with The PCT Shredder and Chemistry for Extraction of Native Protein Compared to Protein Extraction with Mortar and Pestle with Liquid Nitrogen (LNP)
The ProteoSolve-CE NATIVE Plus Reagent is licensed for Research Use Only from Target Discovery, Inc. It is designed to preserve the Native conformation of proteins and their activity, while preventing aggregation and the loss of hydrophobic proteins [3]. Moreover, the reagent design may facilitate the refolding of some denatured proteins, in many cases partially restoring their activity [4].
Features & Benefits: • ProteoSolve-CE Native: A “Gentle” Kit for the Extraction of Native Proteins •
The Kit Contains a Non-detergent Sulphobetaine that Helps Preserve the Native Conformation of Proteins and Their Activity
•
Increase the Number of Unique Proteins on a 2D Gel
•
Use the Synergy of Pressure, Chemistry and Mechanical Force to Optimize Yield
•
Standardize Extraction of Proteins from C. elegans by Pressure Cycling Technology (PCT)
Specifications of the ProteoSolve-CE Native Kit Shredder PULSE Tubes Bottle C: ProteoSolve-CE Native Plus Reagent* Bottle Si: SiC Abrasives
User Manual
*
Set of 12 1 Vial 18 mL 1.2 grams
1 Each
Number of Extractions/Kit Storage Conditions Tissue Sample Amount/Tube
Additional Equipment Required
12 4oC Up to 500 mg The PCT Shredder and Barocycler NEP 3229 or NEP 2320
ProteoSolve-CE Native Plus Reagent was developed and licensed for Research Use Only from Target Discovery, Inc.
For Research Use Only Not for Use in Diagnostic Procedures Note: The ProteoSolve-CE Native Kit works in conjunction with PBI’s pressure generating Barocyclers and The PCT Shredder. Both Barocyclers and the PCT Shredder are sold separately. References: 1. Szewczyk, N.J. et al. (2005) Astrobiology, 5, 690-705. 2. Smejkal, G.B. et al. (2008) HUPO World Congress, Amsterdam, August 16-20, 2008. 3. Vuillard L. et al. (1995) Biochem. J. 305, 337-343. 4. Goldberg, M.E., et al. (1996) Folding & Design 1, 21. 5. The PCT Shredder Manual 6. The NEP2320 or NEP3229 Barocycler Manual
Pressure BioSciences, Inc. 14 Norfolk Ave, South Easton, MA 02375 TEL 508-230-1828 • FAX 508-230-1829 www.pressurebiosciences.com
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