Use the Synergy of Pressure, Chemistry and Mechanical Force to Optimize Yield. ⢠Standardize Extraction of Proteins from C. elegans by Pressure Cycling.
ProteoSolve-CE Native A Pressure-Enhanced Kit for the Extraction of Native Protein from C. elegans The nematode Caenorhabditis elegans is so resistant to lysis that specimens aboard the Space Shuttle Columbia were the only survivors of the disastrous crash [1]. However, using the ProteoSolve-CE Native Kit in combination with The PCT Shredder system and pressure cycling technology (PCT) results in nearly total disruption of the nematodes, including resilient larval stages and worm embryos. The ProteoSolve-CE Native Kit was developed for the maximal recovery of Native proteins and protein complexes from the nematode C. elegans under minimally denaturing conditions in which Native conformation and activity are preserved. Protein yields are greater than those obtained from bead beating or processing with mortar and pestle in the presence of liquid nitrogen. Furthermore, Smejkal et al. [2] showed that bead beating may result in protein loss through denaturation and aggregation.
PCT Shredder (+) PCT
LNP
Extraction of Protein from C. elegans Using PCT in Combination with The PCT Shredder and Chemistry for Extraction of Native Protein Compared to Protein Extraction with Mortar and Pestle with Liquid Nitrogen (LNP)
The ProteoSolve-CE NATIVE Plus Reagent is licensed for Research Use Only from Target Discovery, Inc. It is designed to preserve the Native conformation of proteins and their activity, while preventing aggregation and the loss of hydrophobic proteins [3]. Moreover, the reagent design may facilitate the refolding of some denatured proteins, in many cases partially restoring their activity [4].
Features & Benefits: • ProteoSolve-CE Native: A “Gentle” Kit for the Extraction of Native Proteins •
The Kit Contains a Non-detergent Sulphobetaine that Helps Preserve the Native Conformation of Proteins and Their Activity
•
Increase the Number of Unique Proteins on a 2D Gel
•
Use the Synergy of Pressure, Chemistry and Mechanical Force to Optimize Yield
•
Standardize Extraction of Proteins from C. elegans by Pressure Cycling Technology (PCT)
Specifications of the ProteoSolve-CE Native Kit Shredder PULSE Tubes Bottle C: ProteoSolve-CE Native Plus Reagent* Bottle Si: SiC Abrasives
User Manual
*
Set of 12 1 Vial 18 mL 1.2 grams
1 Each
Number of Extractions/Kit Storage Conditions Tissue Sample Amount/Tube
Additional Equipment Required
12 4oC Up to 500 mg The PCT Shredder and Barocycler NEP 3229 or NEP 2320
ProteoSolve-CE Native Plus Reagent was developed and licensed for Research Use Only from Target Discovery, Inc.
For Research Use Only Not for Use in Diagnostic Procedures Note: The ProteoSolve-CE Native Kit works in conjunction with PBI’s pressure generating Barocyclers and The PCT Shredder. Both Barocyclers and the PCT Shredder are sold separately. References: 1. Szewczyk, N.J. et al. (2005) Astrobiology, 5, 690-705. 2. Smejkal, G.B. et al. (2008) HUPO World Congress, Amsterdam, August 16-20, 2008. 3. Vuillard L. et al. (1995) Biochem. J. 305, 337-343. 4. Goldberg, M.E., et al. (1996) Folding & Design 1, 21. 5. The PCT Shredder Manual 6. The NEP2320 or NEP3229 Barocycler Manual
Pressure BioSciences, Inc. 14 Norfolk Ave, South Easton, MA 02375 TEL 508-230-1828 • FAX 508-230-1829 www.pressurebiosciences.com
Use the Synergy of Pressure, Chemistry and Mechanical Force to Optimize Yield. ⢠Standardize Extraction of Proteins from C. elegans by Pressure Cycling ...
Pressure Cycling Technology Approach to Purify Protein from Lipid-Rich Samples. Proteomic analysis of lipid-rich (adipose) tissue is important in studies of Type ...
yield of protein from yeast suspensions and rat tissues. These findings suggest that .... A benign buffer was used to limit autolysis and clearly differentiate ...
sure Cycling Technology (PCT) based Sample Preparation System (PCT ... PULSE Tubes for downstream analysis, further processing or storage for fu- ture use.
En la carrera de velocidad por equipos participarán dos equipos opuestos de ... Esta competencia individual constará de seis pruebas a lo largo de dos dÃas.
Jun 1, 2002 - ... percent carnauba wax, 45 percent water-white rosin and 10 percent ... The Society for Amateur Scientists (SAS) is a nonprofit research and ...
Vials# 29, 31 â CE grade Water. 4. Vial# 32 â CElixir ... Stop Collecting data at the end of your run, for example 15.0 minutes. Rinse. 10. ... 11. Using pressure, run CEwater through the capillary using Inlet Vial#29 and Outlet. Vial# 10 for 0.5
Industry Classification System (NAICS) and its associated codes. Under ...... platforms are building up the basic infrastructure of the digital economy in a way that can be ..... and Facebook is typically done in the Philippines, where an estimated .
pH changing when moving to the acidic range. Start with the CElixir⢠Accelerator Solution (B) pH 2.5. CElixirâ¢. Accelerator Solution is used as your run buffer ...
generation control systems. The result is devices that provide efficient power conversion, accurate signal conditioning, voltage monitoring and high-resolution.
Polymerase Chain Reaction was performed in a PTC100 thermal cycler (MJ ... 20 ng of template DNA was mixed with 10 pmol of primer (listed in. Table 1), 1X ...
12AVRIL. CLUB EVENT. Domyos Club - Lille. B'twin Village - 4 rue du Professeur Langevin - 59000 Lille. Tel. ... (semaine sportive). DU 30 JUIN. AU 25 AOÛT.
Pressure effect of the electric conductivity was observed and the conductivity increased to 0.5 at log ... temperatures decrease from 3120 K to 1640 K with FeO content. Electron ... 40 µm and embedded on the curette of diamond with pyroxene.
12 juil. 2015 - The mountain bike race for both men and women will be mass starts. Riders will be seeded into a starting grid based on current Union Cycliste ...
Jul 1, 2010 - 03-373. Revised UG-11(d) to include a sentence that compels the vessel ... and the bolt spacing correction factor to Mandatory Appendix 2. ..... aqueous solution at atmospheric pressure is 185°F or higher. ...... 0.1906P â 1000.
their daily health, promote jobs in regions ... of a healthy lifestyle via physical activity as an objective taken up at ... products developed in partnership with host.
May 3, 2002 - Department of Exercise Science, University of South Carolina, Columbia, South Carolina 29208. Received 25 ... 60, and 90% of their individual lactate threshold at four ... imal muscular power did not differ when cycling with.
de performance. • Genres spéculatifs et les mouvements socio-politiques ou les changements culturels (e. g. science-fiction, cybernétique, techno-utopies, etc.) ...
While mainly distillation and absorption methods have been ... using vacuum pressure swing adsorption (VPSA), which delivers the lowest energy consumption ...
The real business cycle model (RBC) model assigns a critical role to ... implement as in Hairault, Langot and Portier [1997], or timeâtoâplan as in .... confidence interval, computed using a Monte Carlo method to sample from the estimated ..... t
Ein informative Broschüre über die Radverkehrspolitik und die. Fahrradnutzung in den Niederlanden. Deutsch: Radfahren in den Niederlanden 2009. Une jolie ...
