(slide 9). (slide 10). ( slirle 11 ). ( slide 12 ). - /1 -. Fir st of all, the rncdium support thP. 111o;;t J i.kely to succeed in displa cing the stnrch gel was obvious]y thP.
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-
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+
1
2
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4
5
6
7
8
9
10
Slide 11: Polyacrylamide gel electrophoresis of wheat gliadins in a vertica.1 Havana (Desaga) system, according to Bushuk. Aluminium lactate buffer, pH 3.1. Varieties: 1-2: Champlein, 3-4: Hardi; 5-6: Top; 7-8: Capitole; 9-10: Clement; 11-12: Florence ...Aurore.
POL YA CRYLAMIDE
GEL
ELECTROPHORESIS
DISADVANTAGES
ADVANTAGES
EASY
TO CAST
OF GLIADINS
AND TO
HANDLE
TOXICITY
OF ACRYLA MIDE
TCA SHORTER
EXPERIMENTAL
AND GRADUAL
S T A-I NI NG T I ME
LOSS
PATTERNS MORE SENSITIVE THE
DETECTION
BANDS
SCANNING
MORE RELIABLE
BETTER RESOLUTION F R.ACT I ON S ALLOWS
OF
. OF ALPHA
TO DIFFERENCIATE
VARIETIES
MORE
OF
STAINED
A ND
I S OE L E CT R I C F OC US I NG
-CARRIED
OUT IN
-PROTEINS
A PH GRADIENT
SEGREGATED
ACCORDING
TO THEIR
ISOELECTRIC
POINTS -PROTEINS -EQUILIBRIUM
CONCENTRATED
IN
VERY SHARP
BANDS
METHOD
'·
i
PH 9
5 1
2
3
4
5
6
7
8
9
10
11
Slide 1i : Isoelectric focusing in polyacrylamide gel of gliadins of several closely related french wheat varieties. pH range: 5 - 9. 1 - Ducat; 2 - Moisson; 3 - Splendeur ;
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