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IUE:N'I'IF'IC!ITION (W l·ifir:A T VAHU:T.l!· :.~ IW 1·:!.VCTI Wl 'lll ye a r ··, th r:- sun110rt n,;1 1

-

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+

1

2

--- .-3

4

5

6

7

8

9

10

Slide 11: Polyacrylamide gel electrophoresis of wheat gliadins in a vertica.1 Havana (Desaga) system, according to Bushuk. Aluminium lactate buffer, pH 3.1. Varieties: 1-2: Champlein, 3-4: Hardi; 5-6: Top; 7-8: Capitole; 9-10: Clement; 11-12: Florence ...Aurore.

POL YA CRYLAMIDE

GEL

ELECTROPHORESIS

DISADVANTAGES

ADVANTAGES

EASY

TO CAST

OF GLIADINS

AND TO

HANDLE

TOXICITY

OF ACRYLA MIDE

TCA SHORTER

EXPERIMENTAL

AND GRADUAL

S T A-I NI NG T I ME

LOSS

PATTERNS MORE SENSITIVE THE

DETECTION

BANDS

SCANNING

MORE RELIABLE

BETTER RESOLUTION F R.ACT I ON S ALLOWS

OF

. OF ALPHA

TO DIFFERENCIATE

VARIETIES

MORE

OF

STAINED

A ND

I S OE L E CT R I C F OC US I NG

-CARRIED

OUT IN

-PROTEINS

A PH GRADIENT

SEGREGATED

ACCORDING

TO THEIR

ISOELECTRIC

POINTS -PROTEINS -EQUILIBRIUM

CONCENTRATED

IN

VERY SHARP

BANDS

METHOD



i

PH 9

5 1

2

3

4

5

6

7

8

9

10

11

Slide 1i : Isoelectric focusing in polyacrylamide gel of gliadins of several closely related french wheat varieties. pH range: 5 - 9. 1 - Ducat; 2 - Moisson; 3 - Splendeur ;

Varieties:

5 - Quest; Florent;

4-

Cappelle;

6 - Trio; 7 - Noroi t; 8 - Top; 9 - Champlein; 10 11 - Gamin.

-~ FUTURE

GLIADINS:

STILL

ELECTROPHORESIS : POLYACRYLAMI DE

----

PERSPECTIVES

THE MOST DISCRIMINANT STARCH

GEL GRADUALLY

PROTEINS REPLACED

BY

POSSIBLE SECOND STEP (IN CASE OF IDENTICAL GLIADIN PATTERNS) I SO ENZYMES WI T H GLUTENIN SUBUNITS IN SDS SYSTEM LATER

ON :

I SOELECTRIC

FOCUSING

?

A~~ LM~vi"~f