Supplementary,File,1.,Isolates,used,in,this,study

T.b.$gambiense,Group,1!

!

!

!

Year,

Host,

Country,

Locality,

1975! 1974! 2004! ! 2004! ! 2004! ! 2004! ! 2002! ! 2002! ! MHOM/CI/02/S14/5/1! 2002! 2002! ! 2000! ! 2000! ! 2000! ! 2002! ! 2000! ! 2000! ! MHOM/CI/84/DAL629! 1984! MHOM/CI/83/DAL607! 1983! MHOM/CI/84/DAL655! 1984! MHOM/CI/83/DAL642! 1983! MHOM/CI/83/DAL403! 1983! MHOM/CI/84/DAL633! 1984! DAL596! 1983! MHOM/CI/83/DAL625! 1983! MHOM/CI/83/DAL595! 1983! 1999! ! 2001! ! 2001! ! 2001! ! 2001! ! 2001! ! 1998! ! 1997! ! MHOM/CI/58/ELIANE! 1952! MHOM/CI/83/DAL543! 1983! MHOM/CI/83/DAL542! 1983! MHOM/CI/84/DAL740! 1984! MHOM/CI/79/THDAL083! 1979! 2002! ! 2002! ! 2002! ! 2002! ! 2002! ! 2002! ! , , , ,

man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man!

Cameroon! Cameroon! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea!

, ,

, ,

ID,

Sample,ID,

Also,known,as…,

1! 2! 3! 4! 5! 6! 7! 8! 9! 10! 11! 12! 13! 14! 15! 16! 17! 18! 19! 20! 21! 22! 23! 24! 25! 26! 27! 28! 29! 30! 31! 32! 33! 34! 35! 36! 37! 38! 39! 40! 41! 42! 43! 44!

BIM_1! MOS! B4_4163P! B4_F303P! B4_G2P! B4_I314P! CB5_1! S1_1_6R! S14_5_1! S15_1_KIVI! S27_16_13! S27_2_6! S3_4_1! S7_2_2! T33_1_7_KIVI! T66_4_2! DEOLA! ISTI! LIGO! LISA! SAKON! SEVAL! TOBO! ZENOU! KIDE! T21_4A! B7_2! N1317_2_KIVI! T4_2_KIVI! AMAN_KIVI! CP1_2_KIVI! YAD! N2561! ELIANE! ABTOR! ADZAM! BRAZO! SETRA! DOB112_KIVI! DOB7_1! LAB13_5! LAB15_2! LAB27_4! LAC11_1!

MHOM/CM/75/BIM! MHOM/CM/74/MOS!

, ,

, ,

Campo! Mbam! Bonon! Bonon! Bonon! Bonon! Bonon! Bonon! Bonon! Bonon! Bonon! Bonon! Bonon! Bonon! Bonon! Bonon! Daloa! Daloa! Daloa! Daloa! Daloa! Daloa! Daloa! Daloa! Gagnoa! Grand8Zathry! Oumé! Oumé! Oumé! Oumé!! Oumé!! Sinfra! Sinfra! Unknown! Vavoua! Vavoua! Vavoua! Vavoua! Boffa! Boffa! Boffa! Boffa! Boffa! Boffa!

! BIIT, test, HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR!

! TgSGP, PCR, +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +!

!

8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8!

, ,

, ,

, ,

, ,

SRA,

,

Sample,ID!

Also,known,as…!

45! 46! 47! 48! 49! 50! 51! 52! 53! 54! 55! 56! 57! 58! 59! 60! 61! 62! 63! 64! 65! 66! 67! 68! 69! 70! 71! 72! 73! 74! 75!

LAC14_3! ! LAC22_11! ! SAC23_3! ! TH1_29_7! ! WAB18_23! ! WAB22_6! ! WAB6_1! ! YEMB3_7! ! LAC22_10! ! SAC1_2! ! YEMB41_2! ! YENB17_4! ! B13_9! ! B15_7! ! B34_2! ! B5_2! ! F10_5! ! F12_20! ! F31_4! ! F34_1! ! F35_2! ! F4_1! ! F55_3! ! F7_6! ! KAC4_10! ! KHOB34_1! ! N4ANNEE_13_KIVI! N70_2! BROB7_16_KIVI! F2_1! KEMB9_5!

! ! !

, ,

Year!

Host!

Country!

Locality!

2002! 2002! 2002! 2002! 2002! 2002! 2002! 2002! 2002! 2002! 2002! 2002! 1998! 1998! 1998! 1998! 1998! 1998! 1998! 1998! 1998! 1998! 1998! 1998! 2002! 2002! 2002! 1998! 2002! 1998! 2002!

man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man! man!

Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea! Guinea!

Boffa! Boffa! Boffa! Boffa! Boffa! Boffa! Boffa! Boffa! Boffa! Boffa! Boffa! Boffa! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka! Dubreka!

BIIT, test! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR! HSR!

TgSGP, PCR! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +! +!

SRA! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8! 8!

T.b.$gambiense,Group,2! ID,

Sample,ID,

Also,known,as…,

Year,

Host,

! Country,

76! 77! 78! 79!

TSW33! KOBIR! OUSOU! TB386!

! MHOM/CI/82/DAL503! MHOM/CI/82/THDAL494! MHOM/CI/78/TH114!

1982! 1982! 1982! 1978!

man! man! man! man!

Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire! Côte!d'Ivoire!

Bouafle! Daloa! Daloa! Koudougou!

! BIIT, test, VHSR! VHSR! VHSR! VHSR!

!

!

!

!

!

!

Year,

Host,

Country,

Locus,

1970! 1961! 1984! 1982!

Fly! man! man! man!

Kenya!! Kenya! Tanzania! Zambia!

Kiboko! Nyanza! Tanzania! Luangwa!

BIIT, test, VHSR! VHSR! VHSR! VHSR!

TgSGP, PCR, 8! 8! 8! 8!

!

!

!

!

!

! TgSGP, PCR, 8! 8!

$ T.b.$rhodesiense! ,

Sample,ID,

80! 81! 82! 83!

K975! N1052! GYBO! Z210!

Also,known,as…, ! ! ! !

$ T.b.$brucei! ,

Sample,ID,

!

Also,known,as…,

Year,

Host,

84! TB927!

TREU927/4!

85! TB247!

STIB247!

1969! Fly! Hartebe 1971! est!

! Locus,

Country,

Locus,

Kenya!

Unknown!

BIIT, test, 8!PCR!

Tanzania!

Serengeti!

HSS!

! TgSGP, PCR, 8! 8! 8! 8!

! For!each!isolate,!the!year!of!isolation,!host,!country!and!location!are!given!along!with!the!results!of! the! BIIT! test! (Blood! Incubation! Infectivity! Test),! which! determines! human! infectivity.! The! presence/absence! of! TgSGP,! the! T.b.( gambiense! Group! 1! human! serum! resistance! gene! and! SRA,! the! T.b.( rhodesiense! human! serum! resistance! gene! are! indicated.! The! majority! of! samples! in! this! study!were!T.b.(gambiense!Group!1,!details!of!which!have!been!previously!published2,46.!

!

!

SRA,

,

8! 8! 8! 8!

! ! ! !

!

!

SRA,

,

+! +! +! +!

! ! ! !

! SRA, 8! 8!

! , ! !