Thursday, 4 June 14h25 Opening address (Christophe Lamaze) 14h30 – 15h15 : Keynote lecture Fulvio Reggiori (University Medical Centre, Utrecht, Netherlands) T01- The phagophore assembly site (PAS) originates from a novel Atg9-positive membranous compartment 15h15 – 16h15 : Session 1 : Autophagie Chairperson: F. Niedergang Joelle Morvan (CRUK London Research Institute; CNRS UMR7156, Strasbourg) T02- In vitro reconstitution of fusion between immature autophagosomes and endosomes Lucile Espert (CPBS, Montpellier) T03- HIV-1 envelope glycoproteins triggers autophagy and cell death in the uninfected CD4 T cells Kevin Moreau (Institut de Biologie, Lille ; Cambridge) T04- Rôle de l'autophagie dans la survie intracellulaire de Yersinia pseudotuberculosis dans les macrophages 16H15 – 16h35 Presentation sponsor 1 Olympus 16h35 – 17h00 : Coffee Break 17h00 – 19h00 Session 2: Actin and membrane trafficking Chairperson: A. Gautreau Dafne Chirivino (Institut Curie, Paris) T05- Interaction of the membrane cytoskeleton linker ezrin with the HOPS complex: a role in endosome maturation Arnaud Labrousse (IPBS, Toulouse) T06- Molecular mechanisms of lysosome mobilisation in phagocytes Sabrina Marion (EMBL ; Institut Cochin, Paris) T07- Ezrin-mediated actin assembly at the phagosome membrane regulates phago-lysosomal fusion. Evelyne Coudrier (Institut Curie, Paris) T08- Role of myosin VI in actin dynamics and melanosome biogenesis Claire Desnos (CNRS FRE 3146 IBPC, Paris) T09- Myosin Va-dependent and independent effects of MyRIP on secretory granule docking to the plasma membrane Arnaud Echard (Institut Curie, Paris) T10- Rab and acto-myosin dependent fission of transport vesicles at the Golgi complex 19h00 - 19h30 Social Get-together 19h30 - 20h30 Dinner 20h30-23h00 Poster session #1 --------------------------------------------------------------------------------------------------------------------------------------------
Friday, 5 June
am
8h30 - 10h10 Session 3 : SNARES Chairperson: François Darchen David Tareste (Institut Jacques Monod, Paris) T11- In vitro membrane interaction and fusion assays to reveal the molecular mechanisms of intracellular fusion machineries Michel Pieren (Université de Lausanne, Suisse) T12- The SM-Protein Vps33 promotes pore opening in vacuole fusion Oussama El Far (Inserm UMR 641, Faculté de Médecine, Marseille) T13- V-ATPase, SNAREs and synaptic transmitter release Sylvette Chasserot-Golaz (INCI, CNRS UPR3212, Strasbourg) T14- S100A10 recruits annexin-A2 to SNARE complexes in adrenergic chromaffin cells during exocytotic stimulation Sandrine Truchet (INRA Jouy-en-Josas) T15- Molecular mechanisms of caseins exocytosis : role of the SNARE proteins 10h10 – 10h30 Coffee Break 10h30- 11h50 Session 4 : Endocytosis : clathrin, actin and caveolae Chairperson: Alexandre Benmerah David Perrais (CNRS UMR5091, Bordeaux) T16- Molecular dynamics of clathrin-mediated endocytosis observed in live cells
Alexis Gautreau (LEBS, CNRS UPR 3082, Gif sur Yvette) T17- Proteo-genomics identifies clathrin in the activation cycle of the Scar/Wave complex Alexandre Grassart (Institut Pasteur, Paris) T18- Pak1 phosphorylation of cortactin enhances its interaction with N-WASP and regulates the clathrin-independent endocytosis used by the IL-2 receptor Bidisha Sinha (Institut Curie, Paris) T19- Response of caveolae to mechanical stress 12h00 - 13h00 Lunch 13h00 - 15h30 Free time
Friday, 5 June
pm
15h30 -17h10 Session 5 : Hijacking of membrane trafficking by viruses Chairperson: Philippe Benaroch Séverine Bär (DKFZ, Heidelberg) T20- Relargage des parvovirus par les cellules infectées: le rôle de la gelsoline. Delphine Muriaux (ENS Lyon) T21- Targeting of retroviral Gag to the plasma membrane is mediated by the phospholipids PIP2 and PS. Marina Caillet (Institut Cochin, Paris) T22- Role of Rab proteins in the formation of HIV-1 particles Julie Mazzolini (Institut Cochin, Paris) T23- Effect of HIV-1 infection on phagocytosis by macrophages : a role for Nef on focal exocytosis Jérôme Bouchet (Institut Cochin, Paris) T24- Lama antibody fragment as an inhibitor of HIV-1 Nef functions in endocytosis 17h10 - 17h30 Coffee Break 17h30 -18h10 Session 6 : Endocytosis and bacteria Chairperson: Sylvie Friant Javier Pizarro-Cerda (Institut Pasteur, Paris) T25- Tetraspanin CD81 is required for recruitment of type II PI4K-alfa during invasion of target cells by Listeria monocytogenes Jost Enninga (Institut Pasteur, Paris) T26- Tracking the dynamic interplay between bacterial and host factors during pathogen-induced vacuolar rupture in real time by fluorescence microscopy 18H10 – 18h30 Presentation sponsor 2: Thermo Fisher 18h30 - 20h30 General Assembly of the Club - Cocktail - posters session #2 20h30 Dinner / Party ---------------------------------------------------------------------------------------------------------------------------------------------
Saturday, 6 June 9h30 - 10h30 Session 6 : Membrane trafficking and immunity Chairperson: Graça Raposo Pauline Gonnord (Institut Curie, Paris) T27- Palmitoylation of Interferon-α receptor subunit IFNAR1 is not essential for receptor trafficking but is required for Jak/Stat signaling Maria-Isabel Yuseff (Institut Curie, Paris) T28- B Cell Polarity in antigen processing and presentation Evelina Gatti (CIML, Marseille) T29- Ubiquitination and MARCH I regulate the intracellular traffic of MHC class II molecules, venting their plasma membrane stabilization in non-activated dendritic cells. 10h50 – 11h10 Coffee Break 11h10- 12h10 Session 7 : Trafficking in neurons and neuroendocrine cells Chairperson: Francis Castets Rémy Sadoul (Inserm U836, Université Joseph Fourier, Grenoble) T30- Exosomes dans le système nerveux central: régulation et rôles possibles Anne-Lise Gaffuri (ESPCI, CNRS UMR7637, Paris) T31- Le trafic et l'adressage axonal du récepteur cannabinoïde CB1 sont conservés chez la drosophile Stéphane Gasman (INCI, CNRS UPR3212, Strasbourg) T32- Molecular mechanisms of secretory granule recycling in neuroendocrine cells: role of scramblase-mediated dynamics of phosphatidylserine. 12h15 Lunch / Departure
