Data Sheet DS101-01-B
multiOPLC 4000 & 8000
W
ork Stations for Purification by High Throughput Parallel Liquid Chromatography
Abstract
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These systems address the needs of organic chemists in drug discovery and medicinal chemistry who need to isolate and characterize compounds of pharmacological interest in large combinatorial chemistry libraries. Scientists who need to screen natural product libraries to identify compounds of interest in crude plant extracts will also benefit from the multiOPLC Work Stations. The multiOPLC 4000 & 8000 Work Stations is designed to process 4 or 8 samples, in parallel and simultaneously, making it possible to purify several hundred* samples per day via reverse or normal phase liquid chromatography, therefore increasing laboratory productivity.
multiOPLC 4000 work station
Design Overview The Bionisis 4- or 8-channel multiOPLC Work Stations are fully integrated sample purification systems that include all of the modules required for effective separation and isolation of compound(s) of interest, including: Quaternary gradient solvent delivery system with degassing and pump Automated sampling system that withdraws a sample from each well from up to 2 standard 96deep well plates Injection via 4 or 8 injection valve loops on the HTSorbTM column that can accommodate 4 or 8 parallel separation channels
BIONISIS
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OPLC separation chamber
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Diode Array Detector to monitor 4 or 8 channels simultaneously
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Fraction collector accommodating 6 standard 96deep well plates to collect the separated compounds easily
Leader in High Throughput Separations
multiOPLC Features and Benefits of the multiOPLC
Increase Sample Throughput with Multiple Channels
Simultaneous chromatographic separation of up to 4 or 8 samples coming from complex matrices in a single run, increasing throughput by a factor of up to 8 relative to standard LC techniques
The Bionisis multiOPLC Work Station includes 4 or 8 discrete channels so that 4 or 8 samples can be separated at a single time on an HTSorb planar sorbent bed without any risk of contamination thanks to the FEW (Flowing Eluent Wall) technology patented by Bionisis.
Up to 50 mg (for the 4 channel version) and 25 mg (for the 8 channel version) of product can be purified per sample per channel Low solvent consumption (up to 100x less than other LC techniques) combined with low column costs and fast separations (5-30 minutes) per run makes this technique particularly economical Preparative chromatography methods are easily adapted from analytical scale runs performed on single channel OPLC instruments (including the OPLC50 and OSU50) Visualization of compounds retained on the stationary phase prevents any loss of information
Fig. 1: multiOPLC4000 and multiOPLC8000 can handle up to 4 or 8 samples respectively
The entire chromatographic process being controlled by a dedicated user-friendly software allows 24 hour a day unattended operation in a GLP environment
Fig. 1bis: Separation of a dye mixture on the multiOPLC 4000
Fig. 2: Separation of Dye Mixture by HTSorb Silica. Mobile phase: Toluene. Flow Rate 1 mL/min (for all 4 channels) 0,24
0,19
0,14 canal 1 DO
canal 2 canal 3 canal 4 0,09
0,04
-0,01 7
12
17
22 tps en min
27
32
37
Typical application of the multiOPLC Work Stations OPLC is a general separation technique that has been successfully applied to problems such as the extraction of compounds of pharmacological interest from natural products, the purification of reaction mixtures to extract the compounds of interest for additional studies (e.g. for NMR or MS) or the isolation of metabolites in biological fluids. Typical examples are shown below.
Purification of crude plant extracts
Purification of reaction mixture after synthesis (a)
(b)
750
400 10.34:2
300 27.39:5
mV
mV
400 %B
150
25.05:4
200
15.11:2
7.11:1
13.05:1
31.53:4 26.24:3
50.23:5 57.01:6
32.08:6
19.46:3
50
20 0.0
20.0
40.0
62.0
Time (min)
(c)
0.0
25.0
1500
62.0
Time (min)
(d) 2000
25.29:2
mV
1000
mV
Fig. 3 : Purification of crude plant extracts (Atractylodes roots) with Bionisis multiOPLC 4000
28.42:3
750
500 33.72:4
29.47:1 5.66:1
23.65:2
37.53:3 41.25:4
36.83:5
49.85:5
75
150 0.0
20.0
40.0
62.0
Time (min)
0.0
20.0
40.0 Time (min)
Fig. 4 : Analysis of plant extracts (Atractylodes roots) with Bionisis multiOPLC 4000
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Fig. 5
S
I
What is OPLC ?
T
E
Optimum Performance Laminar Chromatography™ (OPLC) is a powerful separation technique that employs a planar sorbent bed in a pressurized chamber. The column (HTSorb) is pressurized to 5 MPa (50 bars) and mobile phase is forced through it at constant linear velocity via a pump. Bonded phase and normal phase silica with particles size as low as 5µm are available. OPLC provides superb separation because the design includes the flowing eluent wall (FEW) technology, which provides a linear flow profile. This design eliminates peak broadening and constrains the sample to the separation channels (Figure 5).
62.0
SPECIFICATIONS Dimensions: 690 mm (25.5") H x1045 mm (38.7") W x 630 mm (23.3") D Weight: ~95 kg (209 lbs) Power Requirements: 110/220 V 50/50 Hz, 150 VA All specifications are subject to change as a function of our program of continuous product improvement.
ORDERING INFORMATION multiOPLC 4000, 110/220 V, 50/60 Hz (P/N 400 001) multiOPLC 8000, 110/220 V, 50/60 Hz (P/N 800 001) HTSorb™ - OPLC planar sorbent beds are available with a broad range of stationary phases including Silica for normal phase separations, C18 for Reverse Phase separations, as well as Amino, Cyano, Diol or Chiral for specialized separations. Stationary phases are available in 5 and 11 µm. For a detailed list of HTSorb™ formats that are available, please contact your local Bionisis representative.
BIONISIS S.A. Parc Technologique "Le Carnot" - Hall 9 18-20 Avenue Edouard Herriot 92350 Le Plessis-Robinson, France Tel.: +33 (0)1 40 83 82 00 Fax: +33 (0)1 40 83 82 01 Email:
[email protected] Web: http://www.bionisis.com
BIONISIS
Leader in High Throughput Separations
Copyright Bionisis 2003 – Bionisis, Optimum Performance Laminar Chromatography and HTSorb are trade marks of Bionisis SA
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* Sample throughput depends on the nature of the sample and the purity level objective
